An Adaptive Framework for Real-Time ECG Transmission in Mobile Environments

Wireless electrocardiogram (ECG) monitoring involves the measurement of ECG signals and their timely transmission over wireless networks to remote healthcare professionals. However, fluctuations in wireless channel conditions pose quality-of-service challenges for real-time ECG monitoring services in a mobile environment. We present an adaptive framework for layered coding and transmission of ECG data that can cope with a time-varying wireless channel. The ECG is segmented into layers with differing importance with respect to the quality of the reconstructed signal. According to this observation, we have devised a simple and efficient real-time scheduling algorithm based on the earliest deadline first (EDF) policy, which decides the order of transmitting or retransmitting packets that contain ECG data at any given time for the delivery of scalable ECG data over a lossy channel. The algorithm takes into account the differing priorities of packets in each layer, which prevents the perceived quality of the reconstructed ECG signal from degrading abruptly as channel conditions worsen, while using the available bandwidth efficiently. Extensive simulations demonstrate this improvement in perceived quality

Efficient Error Control for Scalable Media Transmission over 3G Broadcast Networks

Abstract. Broadcast and mobile phone technologies have now combined to provide wireless multimedia services. 3GPP2 has introduced the Broadcast and Multicast Services (BCMCS) architecture in a 3G wireless network. BCMCS are capable of supplying multimedia content, which requires successive frames to arrive within a specific time interval. We analyze the execution time of Reed-Solomon decoding, which is the MAC-layer forward error correction scheme used in cdma2000 1xEV-DO BCMCS, under different air channel conditions. The results show that the time constraints of MPEG-4 cannot be guaranteed by Reed-Solomon decoding when the packet loss rate (PLR) is high, due to its long computation time on current hardware. To alleviate this problem, we propose three error control schemes. Our static scheme bypasses Reed-Solomon decoding at the mobile node to satisfy the MPEG-4 time constraint when the PLR exceeds a given boundary. Our second, dynamic scheme corrects errors in a best-effort manner within the time constraint, instead of giving up altogether when the PLR is high. The third, video-aware dynamic scheme fixes errors in a similar way to the dynamic scheme, but in a priority-driven manner which improves the quality of the final video. Extensive simulation results show the effectiveness of our schemes compared to the original FEC scheme

Acetylation of Surface Lysine Groups of a Protein Alters the Organization and Composition of Its Crystal Contacts

This paper uses crystals of bovine carbonic anhydrase (CA) and its acetylated variant to examine (i) how a large negative formal charge can be accommodated in protein-protein interfaces, (ii) why lysine residues are often excluded from them, and (iii) how changes in the surface charge of a protein can alter the structure and organization of protein-protein interfaces. It demonstrates that acetylation of lysine residues on the surface of CA increases the participation of polar residues (particularly acetylated lysine) in protein-protein interfaces, and decreases the participation of nonpolar residues in those interfaces. Negatively charged residues are accommodated in protein-protein interfaces via (i) hydrogen bonds or van der Waals interactions with polar residues or (ii) salt bridges with other charged residues. The participation of acetylated lysine in protein-protein interfaces suggests that unacetylated lysine tends to be excluded from interfaces because of its positive charge, and not because of a loss in conformational entropy. Results also indicate that crystal contacts in acetylated CA become less constrained geometrically and, as a result, more closely packed (i.e., more tightly clustered spatially) than those of native CA. This study demonstrates a physical-organic approach—and a well-defined model system—for studying the role of charges in protein-protein interactions.Chemistry and Chemical Biolog

Water-Restructuring Mutations Can Reverse the Thermodynamic Signature of Ligand Binding to Human Carbonic Anhydrase

This study uses mutants of human carbonic anhydrase (HCAII) to examine how changes in the organization of water within a binding pocket can alter the thermodynamics of protein–ligand association. Results from calorimetric, crystallographic, and theoretical analyses suggest that most mutations strengthen networks of water-mediated hydrogen bonds and reduce binding affinity by increasing the enthalpic cost and, to a lesser extent, the entropic benefit of rearranging those networks during binding. The organization of water within a binding pocket can thus determine whether the hydrophobic interactions in which it engages are enthalpy-driven or entropy driven. Our findings highlight a possible asymmetry in protein–ligand association by suggesting that, within the confines of the binding pocket of HCAII, binding events associated with enthalpically favorable rearrangements of water are stronger than those associated with entropically favorable ones.Chemistry and Chemical Biolog

Interactions between Hofmeister Anions and the Binding Pocket of a Protein

This paper uses the binding pocket of human carbonic anhydrase II (HCAII, EC 4.2.1.1) as a tool to examine the properties of Hofmeister anions that determine (i) where, and how strongly, they associate with concavities on the surfaces of proteins and (ii) how, upon binding, they alter the structure of water within those concavities. Results from X-ray crystallography and isothermal titration calorimetry show that most anions associate with the binding pocket of HCAII by forming inner-sphere ion pairs with the Zn2+ cofactor. In these ion pairs, the free energy of anion–Zn2+ association is inversely proportional to the free energetic cost of anion dehydration; this relationship is consistent with the mechanism of ion pair formation suggested by the “law of matching water affinities”. Iodide and bromide anions also associate with a hydrophobic declivity in the wall of the binding pocket. Molecular dynamics simulations suggest that anions, upon associating with Zn2+, trigger rearrangements of water that extend up to 8 Å away from their surfaces. These findings expand the range of interactions previously thought to occur between ions and proteins by suggesting that (i) weakly hydrated anions can bind complementarily shaped hydrophobic declivities, and that (ii) ion-induced rearrangements of water within protein concavities can (in contrast with similar rearrangements in bulk water) extend well beyond the first hydration shells of the ions that trigger them. This study paints a picture of Hofmeister anions as a set of structurally varied ligands that differ in size, shape, and affinity for water and, thus, in their ability to bind to—and to alter the charge and hydration structure of—polar, nonpolar, and topographically complex concavities on the surfaces of proteins.Chemistry and Chemical Biolog

Design of a Real-Time Switch with Bounded Delays

Most network switches are designed for best-effort transmission of Internet traffic. Relevant studies have focused on maximizing throughput and minimizing delay in an average sense, but this is not suitable for hard real-time applications, in which guaranteeing delay bound is critical. We propose a real-time switch design, based on a crossbar switching fabric, which combines clearance-time-optimal switching with clock-based scheduling, and we show that any feasible traffic is guaranteed to be switched in two clock periods. The concept of one-shot traffic allows delay to be bounded without a requirement for traffic periodicity, at the cost of a fixed delay of one clock period. The proposed switch uses real-time virtual machine tasks to serve traffic, which simplifies analysis and provides isolation from other system perations. Simulations show that our real-time switch achieves a larger schedulability region and a bounded lower end-to-end delay with a shorter clearance time than iSLIP, which is one of the most widely used crossbar switch schedulers.not peer reviewe

Cross-layer Quality Assessment of Wireless Video Transmission over Mobile Broadcast Networks

The recent development of high-speed data transmission over wireless cellular networks has enabled the delivery of multimedia broadcasting services to mobile users. These services involve a range of interactions among different system components, including the wireless channel, the network, and mobile devices, making it crucial for the service provider to verify the model, design, and behavior of a new service before it is deployed. However, previous studies have largely relied on network simulations or scaled experiments, and there has been little work on the sort of unified framework for qualityof- service (QoS) assessment, which considers the interactions between components, that we propose in this paper. Accurate models of the wireless channel, the network, and the data processing that takes place on a client device, are integrated within our framework, and allow us to predict several key system metrics and the quality of the video stream as it is perceived by users. Furthermore, different models of system components can be easily plugged in to extend this framework. As an example application, we analyze the performance of the process of decoding scalable videos on mobile devices in CDMA2000 wireless cellular networks

Dislocation of polyfocal full-optics accommodative intraocular lens after neodymium-doped yttrium aluminum garnet capsulotomy in vitrectomized eye

We report a case of dislocation of WIOL-CF® polyfocal full-optics intraocular lens (IOL) after neodymium-doped yttrium aluminum garnet (Nd: YAG) laser capsulotomy in the vitrectomized eye. At 22 months before the dislocation of the IOL, a 55-year-old male patient underwent phacoemulsification with WIOL-CF® IOL implantation in a local clinic and 10 months after the cataract surgery the patient underwent pars plana vitrectomy, endolaser photocoagulation and 14% C 3 F 8 gas tamponade for the treatment of rhegmatogenous retinal detachment. At 9 months after the vitrectomy, the patient visited our clinic for a sudden decrease of vision after Nd: YAG capsulotomy in the local clinic. On fundus examination, the dislocated IOL was identified and the Nd: YAG capsulotomy site and the larger break, which is suspected to have been a route of the dislocation were observed in the posterior capsule